Olfactometer studies indicated that the aggregation pheromone of red palm weevil and rhinoceros beetle attracted significantly more number of weevils (13.4 females and 7.6 male weevils) and beetles (6.5 male and 12.3 female beetles), respectively than control. Due to ineffectiveness of the current management practices to control the two important pests on coconut, a study was conducted to know the attractiveness of red palm weevil and rhinoceros beetle to aggregation pheromone. Red palm weevil and Rhinoceros beetle are the major pests inflicting severe damage to coconut palms. These large insects can be detected easily with acoustic sensors, and procedures are being developed to incorporate acoustic technology int.Įfficacy of aggregation pheromone in trapping red palm weevil (Rhynchophorus ferrugineus Olivier) and rhinoceros beetle (Oryctes rhinoceros Linn.) from infested coconut palms.Ĭhakravarthy, A K Chandrashekharaiah, M Kandakoor, Subhash B Nagaraj, D N The coconut rhinoceros beetle, Oryctes rhinoceros, was accidentally introduced into Guam last year and now threatens the Island’s forests and tourist industry. For Permissions, please email: of acoustic sensors to detect coconut rhinoceros beetle in Guam Published by Oxford University Press on behalf of Entomological Society of America. This assay also features an internal positive control to ensure DNA of sufficient quantity and quality is used in the assay, increasing its reliability and reducing the chances of false negative results. Here, we report the development of a multiplex PCR assay targeting cytochrome oxidase I of coconut rhinoceros beetle and oriental flower beetle that can rapidly detect and distinguish between these insects. Eggs, early life stages, and fecal excrement of coconut rhinoceros beetle and oriental flower beetle are morphologically indistinguishable, thereby creating uncertainty when such specimens are discovered in the field. Confounding this program is the widespread presence of another scarab beetle on Oahu, the oriental flower beetle, Protaetia orientalis (Gory and Percheron 1833). An incipient coconut rhinoceros beetle population was recently discovered on the island of Oahu, Hawaii and is currently the target of a large, mutiagency eradication program. The coconut rhinoceros beetle, Oryctes rhinoceros (L.), is a major pest of coconut and other palm trees. The advantage of research is it can be used in integrated pest management (IPM) packages for monitoring of beetle population, and removal of beetles.Ī Multiplex PCR Assay for Differentiating Coconut Rhinoceros Beetle (Coleoptera: Scarabaeidae) From Oriental Flower Beetle (Coleoptera: Scarabaeidae) in Early Life Stages and Excrement. Aggregation pheromone is useful for attracting females. This result indicates the high population of O. rhinoceros and 9.9% other insect species (Rhynchophorus ferrugineus and Xylotrupes gideon). In 12 weeks of monitoring, the traps captured 101 insects consist of 90.1% O. The traps were observed, and the beetles trapped were counted every week. The pheromone compounds were placed in traps (buckets), hung 2 meters above the ground. Aggregation pheromones is a chemical compound containing Ethyl 4-methyl octanoate. rhinoceros that were attracted to aggregation pheromones in the field. The study aimed to monitor the population and analyze the sex ratio of O. rhinoceros attacked one of the coconuts producing villages with more than 75% of the coconut plant population O. Oryctes rhinoceros (Coleoptera: Scarabaeidae) is the most serious pest of coconut plantations in Indonesia. Aggregation pheromones for monitoring the coconut rhinoceros beetle (Oryctes rhinoceros) in Jerukwangi Village, Jepara, Indonesia
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